XRCC1 antibody

Catalog No. ABIN5708404

Product Details anti-XRCC1 Antibody

Target: XRCC1 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XRCC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Antigen affinity purified

Immunogen: A recombinant human partial protein corresponding to amino acids E538-A633 was used as the immunogen for the XRCC1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the XRCC1 antibody should be determined by the researcher.\. Western Blot: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL,Direct ELISA: 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the XRCC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for XRCC1

Target: XRCC1 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1))

Alternative Name: XRCC1 (XRCC1 Products)

Synonyms: xrcc1 antibody, MGC80909 antibody, XRCC1 antibody, rcc antibody, RCC antibody, Xrcc-1 antibody, X-ray repair complementing defective repair in Chinese hamster cells 1 antibody, X-ray repair cross complementing 1 antibody, xrcc1 antibody, XRCC1 antibody, Xrcc1 antibody

Background: XRCC1 (X-ray Repair Complementing Defective in Chinese Hamster 1) is a DNA repair protein which complexes with DNA ligase III. The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. The XRCC1 gene is mapped to 19q13.31. The XRCC1 interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. XRCC1 is phosphorylated in vivo and in vitro by CK2, and CK2 phosphorylation of XRCC1 on ser518, thr519, and thr523 largely determines aprataxin binding to XRCC1 through its FHA domain.

UniProt: P18887

Pathways: DNA Damage Repair