LIG1 antibody (Internal Region)
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- Target See all LIG1 Antibodies
- LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This LIG1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Purpose
- LIG1 / DNA ligase I
- Sequence
- RVREDKQPEQ ATTS
- Cross-Reactivity
- Human, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-RVREDKQPEQATTS, from the internal region (near C-Terminus) of the protein sequence according to NP_000225.1.
- Isotype
- IgG
- Top Product
- Discover our top product LIG1 Primary Antibody
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- Application Notes
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Western Blot: Approx 125 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102 kDa according to NP_000225.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:16000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))
- Alternative Name
- LIG1 (LIG1 Products)
- Synonyms
- ATLIG1 antibody, DNA ligase 1 antibody, T6D22.23 antibody, ligI antibody, AL033288 antibody, LigI antibody, lig1 antibody, wu:fc54a11 antibody, DNA ligase 1 antibody, DNA ligase 1, putative antibody, ligase I, DNA, ATP-dependent L homeolog antibody, ligase I, DNA, ATP-dependent antibody, LIG1 antibody, PB000674.02.0 antibody, PTRG_06243 antibody, PKH_140260 antibody, lig1.L antibody, Lig1 antibody, lig-1 antibody, lig1 antibody
- Background
- LIG1, ligase I, DNA, ATP-dependent, MGC117397, MGC130025, DNA ligase I
- Gene ID
- 3978, 81513
- NCBI Accession
- NP_000225
- Pathways
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
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