AIM2 antibody (C-Term)

Catalog No. ABIN571131

This anti-AIM2 antibody is a Goat Polyclonal antibody detecting AIM2 in WB, ELISA, IHC, IF and FACS. Suitable for Human.

Quick Overview for AIM2 antibody (C-Term) (ABIN571131)

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AIM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-AIM2 Antibody

Binding Specificity: C-Term

Purpose: AIM2

Sequence: GEKLQLTSGV HSTIK

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GEKLQLTSGVHSTIK, from the C Terminus of the protein sequence according to NP_004824.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 37 kDa band observed in lysates of cell line HeLa (calculated MW of 39.0 kDa according to Human NP_004824.1). Recommended concentration: 0.5-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AIM2

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Alternative Name: AIM2

Background: AIM2, absent in melanoma 2, PYHIN4

Molecular Weight: 39.0kDa

Gene ID: 9447

NCBI Accession: NP_004824

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome