NFAT1 antibody (AA 729-743)

Catalog No. ABIN574389

Product Details anti-NFAT1 Antibody

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Binding Specificity: AA 729-743

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This NFAT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NFATC2. This antibody is expected to recognize all three reported isoforms (NP_036472.2, NP_775114.1, NP_001129493.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Mouse, Rat, Panda, Bovine, Dog, Bat, Horse, Pig (93%) Elephant, Rabbit, Opossum (87%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QQFRTGLSSPDARYQ from an internal region of human NFATC2 (NP_036472.2, NP_775114.1, NP_001129493.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Mouse, Rat, Panda, Bovine, Dog, Bat, Horse, Pig (93%), Elephant, Rabbit, Opossum (87%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:64000), WB (0.3 - 1 μg/mL)

Usage: Western Blot: Approx 110kD band observed in Human lysates of cell line MOLT4 (calculated MW of 100kD according to NP_775114.1). Recommended concentration: 0.3-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NFAT1

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Alternative Name: NFAT1 / NFATC2 (NFAT1 Products)

Synonyms: NFATC2 antibody, AI607462 antibody, NF-ATc2 antibody, NFAT1 antibody, NFAT1-D antibody, Nfatp antibody, NFATP antibody, nuclear factor of activated T-cells 2 antibody, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 antibody, NFATC2 antibody, nfatc2 antibody, Nfatc2 antibody

Background: Name/Gene ID: NFATC2

Synonyms: NFATC2, NF-ATc2, NF-ATp, NFAT1, NFAT pre-existing subunit, NFATP

Gene ID: 4773

NCBI Accession: NP_036472, NP_775114, NP_001129493

UniProt: Q13469

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, VEGF Signaling, BCR Signaling