AIF antibody (AA 98-609)

Catalog No. ABIN5776052

The Mouse Monoclonal anti-AIF antibody (Clone AT22E9) (ABIN5776052) specifically detects AIF in WB, ELISA, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for AIF antibody (AA 98-609) (ABIN5776052)

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AIF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: AT22E9

Product Details anti-AIF Antibody

Binding Specificity: AA 98-609

Purpose: Human AIF/AIFM1 antibody

Purification: Purified

Immunogen: Recombinant human AIFM1 (98-609aa) purified from E. coli

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) with 0.02 % Sodium Azide, 10 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details for AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: AIF/AIFM1

Background: Apoptosis-inducing factor 1, also known as AIFM1, is a mitochondrial protein that translocates to the nucleus upon induction of apoptosis. AIFM1 has been shown to cause DNA fragmentation and chromatin condensation and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression has been shown to prevent the release of AIFM1 from mitochondria, but not to block its apoptogenic activity. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy.

NCBI Accession: NP_665811

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect