IL12 antibody

Catalog No. ABIN596134

This Goat Polyclonal antibody specifically detects IL12 in ELISA, WB and Neut. It exhibits reactivity toward Dog.

Quick Overview for IL12 antibody (ABIN596134)

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This IL12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Neutralization (Neut)

Product Details anti-IL12 Antibody

Specificity: This antibody recognizes both the p35 and p40 sub-units of canine IL-12. Cross-reactivity (direct ELISA): ~40 % with recombinant human IL-12 p35/p40 and recombinant feline IL-12 p35/p40, <10 % with recombinant rat IL-12 p35/p40.

Purification: Affinity purified

Sterility: 0.2 μm filtered

Immunogen: CHO-derived recombinant canine interleukin 12 p40 (aa23-329, Q28268) and p35 (aa 26-222, Q28267).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Neut, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Neutralization. ELISA (capture): 0.2-0.8 μg/mL. Western Blot: 0.1-0.2 μg/mL. Detection limit for rcaIL-12 is ~50 ng/lane under non-reducing and reducing conditions. Neutralization: The ND 50 was determined to be ~0.1-0.5 μg/mL in the presence of 25 ng/mL of rcaIL-12, using PHA activated human peripheral blood mononuclear cells (PBMCs).

Comment:

Target Species of Antibody: Dog

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS, 40-50 % glycerol

Concentration: Lot specific

Buffer: Lyophilized from 0.2 um filtered solution in PBS, 5 % trehalose

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for IL12

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL12

Background: Name/Gene ID: IL-12

Synonyms: IL-12

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome