IKK alpha antibody (Ser180, Ser181)

Catalog No. ABIN601601

The Rabbit Polyclonal anti-IKK alpha antibody is suitable to detect IKK alpha in samples from Human, Mouse, Rat, Cow, Pig, Dog, Monkey, Rabbit, Horse, Chicken and Xenopus laevis. It has been validated for WB, IHC and ELISA.

Quick Overview for IKK alpha antibody (Ser180, Ser181) (ABIN601601)

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Monkey, Rabbit, Horse, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKK alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-IKK alpha Antibody

Binding Specificity: Ser180, Ser181

Specificity: Recognizes human IKK alpha, beta when phosphorylated at serine 180/181. Species cross-reactivity: mouse, rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide corresponding to human IKKa/b around the phosphorylation site of serine 180/181 (C-T-SP-F-V ).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:4000), IHC (1:50 - 1:100), WB (1:500 - 1:1000)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 1:4000. Western Blot: 1:500-1:1000. Immunohistochemistry: 1:50-1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS (without Mg2+, Ca2+),  pH 7.4, 150 mM sodium chloride, 0.02 % sodium azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for IKK alpha

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Alternative Name: CHUK / IKKA / IKK Alpha

Background: Name/Gene ID: CHUK
Subfamily: IKAPPAB
Family: Protein Kinase

Synonyms: CHUK, I-kappa-B kinase alpha, I-kappa-B kinase-alpha, Ikb kinase alpha, IKK-A, IKBKA, IKK1, NFKBIKA, I-kappa-B kinase 1, IkappaB kinase, IKK Alpha, IKK-alpha, IKKA, Transcription factor 16, IkappaBalpha Kinase, IkB kinase alpha subunit, IKK-a kinase, Ikkalpha, TCF-16, TCF16

Gene ID: 1147

UniProt: O15111

Pathways: PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins