Muscarinic Acetylcholine Receptor M2 antibody (AA 168-192)

Catalog No. ABIN601952

Product Details anti-Muscarinic Acetylcholine Receptor M2 Antibody

Target: Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))

Binding Specificity: AA 168-192

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Monkey, Guinea Pig, Sheep, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Muscarinic Acetylcholine Receptor M2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes the pentapeptide VRTVE (aa 168-172) corresponding to the N-terminal part of the second extracellular loop of the human M2 receptor. Species cross-reactivity: rat, mouse and guinea pig.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Sheep, Panda, Dog, Bovine, Horse, Pig, Guinea pig (100%) Elephant, Bat, Rabbit (96%) Hamster, Opossum, Platypus (92%) Xenopus (88%) Stickleback, Pufferfish, Zebrafish (84%) Turkey, Chicken (80%).

Purification: Protein G purified

Immunogen: Synthetic peptide corresponding to the second extracellular loop aa168-192, VRTVEDGECYIQFFSNAAVTFGTAI(C) of human muscarinic acetylcholine receptor M2. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Sheep, Panda, Dog, Bovine, Horse, Pig, Guinea pig (100%), Elephant, Bat, Rabbit (96%), Hamster, Opossum, Platypus (92%), Xenopus (88%), Stickleback, Pufferfish, Zebrafish (84%), Turkey, Chicken (80%).

Type of Immunogen: Synthetic peptide

Clone: B8E5

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (1:50), IF (1:50), IHC, IHC-Fr (1:50), WB (1:50)

Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. ELISA: 1:50. Western Blot: 1:50 under non-reducing conditions. Immunohistochemistry (frozen): 1:50. Immunofluorescence: 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % BSA

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for Muscarinic Acetylcholine Receptor M2

Target: Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))

Alternative Name: CHRM2 / M2 (CHRM2 Products)

Synonyms: HM2 antibody, AChR-M2 antibody, Chrm-2 antibody, M2 antibody, chrm2 antibody, Acm2 antibody, GPM2 antibody, cholinergic receptor muscarinic 2 antibody, cholinergic receptor, muscarinic 2, cardiac antibody, cholinergic receptor, muscarinic 2a antibody, cholinergic receptor, muscarinic 2 antibody, CHRM2 antibody, Chrm2 antibody, chrm2a antibody

Background: Name/Gene ID: CHRM2
Subfamily: Acetylcholine
Family: GPCR

Synonyms: CHRM2, 7TM receptor, HM2, M2 muscarinic receptor, M2-mAChR, Muscarinic receptor M2, Muscarinic M2 receptor

Gene ID: 1129

UniProt: P08172