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CD28 antibody (Biotin)

CD28 Reactivity: Human FACS, ELISA, Func Host: Mouse Monoclonal ANC28-1-5D10 Biotin

Catalog No. ABIN610013

Datasheet as PDF

Target See all CD28 Antibodies

CD28
Reactivity
171

117

57

38

21

20

12

12

7

7

6

5

1

1

1

1
Human
Host
123

78

23

16

8

3

2

1
Mouse
Compatible Secondaries
Clonality
174

74

3
Monoclonal
Conjugate
85

29

27

18

12

5

5

5

4

4

4

4

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1
This CD28 antibody is conjugated to Biotin
Application
189

93

47

46

37

29

27

26

19

17

13

7

6

6

4

4

4

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), ELISA, Functional Studies (Func)

Specificity

CD28 expressed on T cells and also on BHK cells transfected with human CD28. This antibody has been characterized as a superagonist.

Purification

Protein A purified

Immunogen

Recombinant Human CD28 and HPB-MLT human tumor cells.

Type of Immunogen: Recombinant protein

Clone

ANC28-1-5D10

Isotype

IgG1 kappa

Top Product

Discover our top product CD28 Primary Antibody
anti-CD28 (CD28) (AA 1-120) antibody
CD28 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-CD28 (CD28) antibody
CD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) antibody (FITC)
CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 FITC

anti-CD28 (CD28) (AA 19-152) antibody
CD28 Reactivity: Human FACS, ELISA Host: Rabbit Monoclonal DM61 unconjugated Recombinant Antibody

anti-CD28 (CD28) antibody
CD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) antibody
CD28 Reactivity: Rat FACS, IP Host: Mouse Monoclonal JJ319 unconjugated

anti-CD28 (CD28) (C-Term) antibody
CD28 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-CD28 (CD28) antibody (PE)
CD28 Reactivity: Rat FACS Host: Mouse Monoclonal JJ319 PE

anti-CD28 (CD28) (Internal Region) antibody
Verified CD28 Reactivity: Human WB, ELISA, IHC Host: Goat Polyclonal unconjugated

anti-CD28 (CD28) antibody (APC)
CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 APC

Application Notes

Approved: ELISA, Flo, Func

Usage: Function: ANC28.1 stimulates expression of IL-2 from CD28+ cells. Divalent ANC28.1 can induce Antigen Independent T cell proliferation. In combination with anti-CD81 mAb, it can prime naive T cells to acquire effector function independent of TCR ligation. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Not recommended for: WB

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 5 % Glycerol, 0.2 % BSA, 0.04 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Do not freeze.

Storage

4 °C

Storage Comment

Store at 4°C. Do not freeze.
Target

CD28

Alternative Name

CD28 (CD28 Products)

Synonyms

Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody

Background

Name/Gene ID: CD28

Synonyms: CD28, CD28 antigen, CD28 antigen (Tp44), Tp44, CD28 Molecule

Gene ID

940

UniProt

P10747

Pathways

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response