CEACAM5 antibody
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- Target See all CEACAM5 Antibodies
- CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))
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Reactivity
- Human, Monkey
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CEACAM5 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-CD66 / CEA (CEA31), Purified, BSA-free
- Characteristics
- This antibody recognizes proteins of 80-200 kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA ) from pleural mesotheliomas (rarely or weakly CEA ). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Purification
- Purified
- Immunogen
- Human colon carcinoma extract
- Clone
- CEA31
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CEACAM5 Primary Antibody
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- Application Notes
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Immunohistology formalin-fixed 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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MCF7 or 293T cells. Colon carcinoma
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS (no BSA, no azide)
- Preservative
- Azide free
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- Target
- CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))
- Alternative Name
- CD66 (CEA) (CEACAM5 Products)
- Synonyms
- CD66e antibody, CEA antibody, CEACAM5 antibody, CEACAM antibody, 1600029H12Rik antibody, Psg30 antibody, carcinoembryonic antigen related cell adhesion molecule 5 antibody, carcinoembryonic antigen antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, carcinoembryonic antigen-related cell adhesion molecule 8 antibody, carcinoembryonic antigen gene family antibody, CEACAM5 antibody, Ceacam5 antibody, LOC100409241 antibody, Cea antibody
- Background
- Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1)
- Molecular Weight
- 80-200 kDa
- Gene ID
- 1048, 634, 709196
- UniProt
- P06731
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