Hepatocyte Specific Antigen antibody (CF®568)

Catalog No. ABIN6152822

Product Details anti-Hepatocyte Specific Antigen Antibody (CF®568)

Target: Hepatocyte Specific Antigen (HepPar-1)

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hepatocyte Specific Antigen antibody is conjugated to CF®568

Application: Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Purpose: Mouse Monoclonal anti-Hepatocyte-Specific (CPS1/1022), CF568 Conjugate

Characteristics: This MAb recognizes a protein of 165 kDa, identified as carbamoyl phosphate synthetase 1 (CPS1). This mitochondrial enzyme catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells.Deficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia. CPS1 is a hepatocyte specific protein that localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC s occur primarily in the stomach, but they are also found in many other organs. CPS1 may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of CPS1 correlates with smaller tumor size and longer patient survival. Occasionally, CPS1 is also found in gastric carcinomas as well as in a few other non-hepatic tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CPS1 protein

Clone: CPS1-1022

Isotype: IgG1

Application Details

Application Notes: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1.0 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HeLa cells. Liver or Hepatocellular Carcinoma (HCC)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Hepatocyte Specific Antigen

Target: Hepatocyte Specific Antigen (HepPar-1)

Alternative Name: Hepatocyte-Specific (HepPar-1 Products)

Background: Carbamoyl-phosphate synthetase 1 (CPS1), Carbamoylphosphate synthetase 1, CPSase 1, CPSASE1

Molecular Weight: ~165 kDa

Gene ID: 1373, 149252

UniProt: P31327