Blood Group Lewis Y antibody (CF®568)
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- Target
- Blood Group Lewis Y
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- CF®568
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
- Purpose
- Mouse Monoclonal anti-Lewis-Y (A70-A/A9), CF568 Conjugate
- Characteristics
- This antibody recognizes a carbohydrate epitope present on tumor-associated Lewis Y antigen (Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta). Lewis Y is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis Y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Live Ls174T cells (human colon carcinoma cell line)
- Clone
- A70-A-A9
- Isotype
- IgG1 kappa
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- Application Notes
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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Lung, Colon, Bladder or Hepatocellular Carcinoma
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- Blood Group Lewis Y
- Alternative Name
- Lewis-Y
- Background
- Lewis Y antigen
- Molecular Weight
- Multiple
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