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Melanoma Marker antibody (CF®660R)

Reactivity: Human, Dog, Mouse IHC, IF Host: Mouse Monoclonal PNL2 CF®660R
Catalog No. ABIN6157189
  • Target See all Melanoma Marker products
    Melanoma Marker
    Reactivity
    Human, Dog, Mouse
    Host
    • 48
    Mouse
    Clonality
    • 48
    Monoclonal
    Conjugate
    • 9
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    This Melanoma Marker antibody is conjugated to CF®660R
    Application
    • 29
    • 18
    • 18
    • 18
    • 1
    Immunohistochemistry (IHC), Immunofluorescence (IF)
    Purpose
    Mouse Monoclonal anti-Melanoma Marker (PNL2), CF660R Conjugate
    Characteristics
    Anti-PNL2 is a novel monoclonal antibody, which has recently been introduced as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. The antigen recognized by PNL2 is different from Melan A and gp100. Its epitope is not destroyed by digestion with neuraminidase i.e. its epitope id not glycosylated. Anti-PNL2 may be most useful because of its high sensitivity for metastatic melanoma (87 % ), as opposed to 76 % for anti-HMB45 and 82 % for anti-MART-1. Anti-PNL2 labels intra-epidermal nevi while the dermal component of compound nevi are largely non-reactive with anti-PNL2. Antibodies against PNL2, MART-1 (Melan A) and HMB45 stain most clear cell sarcoma cells and a few cells in angio-myolipomas and lymphangioleiomyomatosis. Anti-PNL2 is a useful antibody for the identification of melanomas and clear cell sarcomas. Differential diagnosis is aided by the results from a panel of antibodies, including antibodies against HMB45, MART-1, tyrosinase, and MiTF. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the < a href="https://biotium.com/wp-content/uploads/2013/07/CF-dye-selection-guide.pdf" target="_blank" rel="noopener">CF® Dye Selection Guide for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Melanocyte antigen
    Clone
    PNL2
    Isotype
    IgG1
  • Application Notes
    Immunohistology (formalin)
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    Melanoma

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    Melanoma Marker
    Abstract
    Melanoma Marker Products
    Background
    Human Melanoma Associated Antigen, PNL2
    Molecular Weight
    Multiple (160 kDa, 100 kDa and <10 kDa)
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