Topoisomerase II alpha antibody (AA 1352-1493) (Biotin)
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- Target See all Topoisomerase II alpha (TOP2A) Antibodies
- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
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Binding Specificity
- AA 1352-1493
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Topoisomerase II alpha antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- Mouse Monoclonal anti-Topoisomerase-II-alpha (TOP2A/1362), Biotin Conjugate
- Characteristics
- This antibody recognizes a 170 kDa protein, which is identified as topoisomerase IIα. It shows no cross-reaction with topoisomerase IIβ or topoisomerase I. Topo IIα plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIα is also implicated in drug resistance of tumor cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human Topoisomerase II alpha fragment (aa1352-1493) (exact sequence is proprietary)
- Clone
- TOP2A-1362
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product TOP2A Primary Antibody
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- Application Notes
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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HeLa Cells or Tonsil
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
- Alternative Name
- Topoisomerase-II-alpha (TOP2A Products)
- Synonyms
- TOP2 antibody, TP2A antibody, TOP2A antibody, Top-2 antibody, TOP-2 antibody, F12B17.110 antibody, F12B17_110 antibody, thimet metalloendopeptidase 2 antibody, fc08c07 antibody, wu:fc08c07 antibody, DNA topoisomerase II alpha antibody, DNA topoisomerase II, alpha isozyme antibody, topoisomerase (DNA) II alpha antibody, Zincin-like metalloproteases family protein antibody, TOP2A antibody, Bm1_08705 antibody, Top2a antibody, AT5G10540 antibody, top2a antibody
- Molecular Weight
- 170 kDa
- Gene ID
- 7153, 156346
- UniProt
- P11388
- Pathways
- Cell Division Cycle, Mitotic G1-G1/S Phases
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