Thrombomodulin antibody (AA 69-194) (CF®594)
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- Target See all Thrombomodulin (THBD) Antibodies
- Thrombomodulin (THBD)
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Binding Specificity
- AA 69-194
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Thrombomodulin antibody is conjugated to CF®594
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat), Immunostaining (ISt)
- Purpose
- Mouse Monoclonal anti-Thrombomodulin / CD141 (THBD/1591), CF594 Conjugate
- Characteristics
- This antibody recognizes a protein of 75 kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human Thrombomodulin protein fragment (aa69-194) (exact sequence is proprietary)
- Clone
- THBD-1591
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product THBD Primary Antibody
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- Application Notes
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Immunohistology (formalin) 1-2 μg/mL
- ELISA 2-4 μg/mL for coating (order Ab without BSA)
- pH 9.0
- for 10-20 min followed by cooling at RT for 20 min
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris buffer with1 mM EDTA
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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THP1 Cells. Placenta, Angiosarcoma, Bladder or Cervical Carcinoma
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- Thrombomodulin (THBD)
- Alternative Name
- Thrombomodulin / CD141 (THBD Products)
- Synonyms
- AHUS6 antibody, BDCA3 antibody, CD141 antibody, THPH12 antibody, THRM antibody, TM antibody, AI385582 antibody, THBD antibody, HAST antibody, HAST3 antibody, M-PST antibody, ST1A3/ST1A4 antibody, ST1A5 antibody, STM antibody, TL-PST antibody, thrombomodulin antibody, sulfotransferase family 1A member 3 antibody, THBD antibody, Thbd antibody, SULT1A3 antibody
- Background
- AHUS6, BDCA3, CD141, Fetomodulin, Thbd, THPH12, THRM, Thrombomodulin (TM)
- Molecular Weight
- 100 kDa
- Gene ID
- 7056, 2030
- UniProt
- P07204
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