EpCAM antibody (Extracellular Domain)

Catalog No. ABIN6158700

Product Details anti-EpCAM Antibody

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Binding Specificity: AA 77-202, Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Coating (Coat)

Purpose: Mouse Monoclonal anti Ep-CAM / CD326 (HEA125)

Characteristics: This antibody recognizes a 40-43 kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. It has been reported that this epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative.

Purification: Purified

Immunogen: Recombinant human EpCAM protein fragment from the extracellular domain (aa77-202) (exact sequence is proprietary)

Clone: HEA125

Isotype: IgG2b kappa

Application Details

Application Notes: ELISA 2-4 μg/mL for coating (order Ab without BSA)

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

HT29 cells or breast tumor

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Preservative: Azide free

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: Ep-CAM / CD326 (EPCAM Products)

Synonyms: DIAR5 antibody, EGP-2 antibody, EGP314 antibody, EGP40 antibody, ESA antibody, HNPCC8 antibody, KS1/4 antibody, KSA antibody, M4S1 antibody, MIC18 antibody, MK-1 antibody, TACSTD1 antibody, TROP1 antibody, ECS-1 antibody, ECS1 antibody, ESA1 antibody, M17S1 antibody, EGP antibody, Ep-CAM antibody, sb:cb6 antibody, tacstd antibody, wu:fj17g02 antibody, zgc:110304 antibody, zgc:77119 antibody, tacstd1 antibody, MGC80540 antibody, EPCAM antibody, egp antibody, ksa antibody, m4s1 antibody, mk-1 antibody, cd326 antibody, egp40 antibody, mic18 antibody, trop1 antibody, ep-cam antibody, hegp-2 antibody, co17-1a antibody, ga733-2 antibody, CD326 antibody, Egp314 antibody, EpCAM1 antibody, GA733-2 antibody, Ly74 antibody, Tacsd1 antibody, Tacstd1 antibody, gp40 antibody, epithelial cell adhesion molecule antibody, flotillin 2 antibody, epithelial cell adhesion molecule S homeolog antibody, EPCAM antibody, FLOT2 antibody, epcam antibody, epcam.S antibody, Epcam antibody

Background: Adenocarcinoma-associated Antigen| Cell Surface Glycoprotein Trop-1| EGP2| EGP314| EGP40| Epithelial Cell Adhesion Molecule| Epithelial Glycoprotein 314| ESA| KSA| TACD1| TROP1| Tumor-associated Calcium Signal Transducer 1 (TACSTD1)| ECS-1| Epidermal Surface Antigen 1| ESA1| FLOT2| Flotillin-2| Membrane Component| Chromosome 17| Surface Marker-1 (M17S1)| REG-1| Reggie-1| Reggie-2

Molecular Weight: 40-43 kDa

Gene ID: 4072, 542050

UniProt: P16422