CD47 antibody (CF®488A)
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- Target See all CD47 Antibodies
- CD47
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Reactivity
- Human, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD47 antibody is conjugated to CF®488A
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-CD47 (B6H12.2), CF488A Conjugate
- Characteristics
- This antibody reacts with Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Intact CD47 purified from placenta
- Clone
- B6H12-2
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD47 Primary Antibody
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- Application Notes
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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HUVEC, OVCA3 cells, Placenta, Brain and Ovarian Tumors
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- CD47
- Alternative Name
- CD47 (CD47 Products)
- Synonyms
- IAP antibody, MER6 antibody, OA3 antibody, 9130415E20Rik antibody, AA407862 antibody, AI848868 antibody, AW108519 antibody, B430305P08Rik antibody, Itgp antibody, CD47/IAP antibody, alkaline phosphatase, intestinal antibody, CD47 molecule antibody, CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody, Cd47 molecule antibody, ALPI antibody, CD47 antibody, Cd47 antibody
- Background
- Antigenic Surface Determinant Protein OA3, IAP, Integrin Associated Protein, Integrin Associated Signal Transducer, MER6, OA3, Protein MER6, Rh Related Antigen
- Molecular Weight
- ~50 kDa
- Gene ID
- 961, 446414
- UniProt
- Q08722
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