VEGFB antibody (AA 21-188)

Catalog No. ABIN616026

Product Details anti-VEGFB Antibody

Target: VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

Binding Specificity: AA 21-188

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGFB antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects VEGF-B.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein-A purified

Immunogen: Highly pure (>95%) recombinant human VEGF-B167 (Ala21-Arg188) derived from E. coli

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for up to 1 month. Following reconstitution antibody can be stored at 2-8 °C for up to two weeks or (in aliquots) at -20 °C for longer.

Target Details for VEGFB

Target: VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

Alternative Name: VEGF-B (VEGFB Products)

Synonyms: VEGFL antibody, VRF antibody, VEGF-B antibody, Vrf antibody, vascular endothelial growth factor B antibody, VEGFB antibody, Vegfb antibody

Background: Vascular endothelial growth factor B (VEGF-B), also known as vascular endothelial growth factor-related factor (VRF), is a member of the VEGF family of growth factors that share structural and functional similarity (1, 2). Five mammalian members, including VEGF-A, B, C, D and PlGF, have been identified. VEGF family members are disulfide-linked dimeric proteins that are important regulators of physiological and pathological vasculogenesis, angiogenesis and lymphangiogenesis. VEGF-B is expressed in most tissues, especially in heart, skeletal muscle and pancreas. In many tissues, VEGF-B is coexpressed and can heterodimerize with VEGF (3). By alternative splicing, two isoforms of mature VEGF-B containing 167 or 186 amino acid (aa) residues exist (3, 4). The two VEGF-B isoforms have identical amino-terminal cysteine knot VEGF homology domains but the carboxyl end of VEGF-B167 differs from that of VEGF-B186 by the presence of a highly basic cysteine-rich heparin binding domain. Whereas VEGF-B186 is a secreted diffusible protein, VEGF-B167 is sequestered into the cell matrix after secretion. Both VEGF-B isoforms bind VEGF receptor 1 (VEGFR-1), but not VEGFR-2 or VEGFR-3 (5). On endothelial cells, ligation of VEGFR-1 by VEGF-B has been shown to regulate the expression and activity of urokinase type plasminogen activator and plasminogen activator inhibitor 1. VEGF-B167 and a proteolytically processed form of VEGF-B186 (VEGF-B127) also bind neuropilin1 (NP1), a type I transmembrane receptor for semaphorins/collapsins, ligands involved in neuron guidance (6). Besides VEGF-B, NP1 has been shown to bind PlGF-2, VEGF165 and VEGFR-1 (6, 7). The many interactions of NP1 with VEGF ligands and receptor suggest that NP1 may function as a regulator of angiogenesis (7).Synonyms: VEGF-related factor, VEGFB, VRF, Vascular endothelial growth factor B

Gene ID: 7423

NCBI Accession: NP_003368

UniProt: P49765

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals