Cytokeratin 8/18 antibody (Biotin)

Catalog No. ABIN6161918

Product Details anti-Cytokeratin 8/18 Antibody (Biotin)

Target: Cytokeratin 8/18

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 8/18 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Purpose: Mouse Monoclonal anti-Cytokeratin 8/18 (KRT8/803 + KRT18/835), Biotin Conjugate

Characteristics: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immuno-histochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human KRT8 protein (KRT8/803), Recombinant full-length human KRT18 protein (KRT18/835)

Clone: KRT8-803 KRT18-835

Isotype: IgG

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1.0 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1.0 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Cytokeratin 8/18

Target: Cytokeratin 8/18

Abstract: Cytokeratin 8/18 Products

Background: P145, Steel Factor Receptor, Stem Cell Factor Receptor (SCF-Receptor), Mast Cell Growth Factor Receptor

Molecular Weight: 52.5 kDa (CK8), 45 kDa (CK18)

Gene ID: 3856, 3875