Podoplanin antibody (AA 24-126) (CF®640R)
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- Target See all Podoplanin (PDPN) Antibodies
- Podoplanin (PDPN)
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Binding Specificity
- AA 24-126
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Podoplanin antibody is conjugated to CF®640R
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- Purpose
- Mouse Monoclonal anti-Podoplanin (PDPN/1433), CF640R Conjugate
- Characteristics
- This antibody recognizes a muco-protein of 38-43 kDa, which is identified Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human Podoplanin (PDPN) protein fragment (aa24-126) (exact sequence is proprietary)
- Clone
- PDPN-1433
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product PDPN Primary Antibody
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- Application Notes
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL Western blotting 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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HeLa Cells. Angiosarcoma or Mesothelioma.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- Podoplanin (PDPN)
- Alternative Name
- Podoplanin (PDPN Products)
- Synonyms
- PDPN antibody, AGGRUS antibody, GP36 antibody, GP40 antibody, Gp38 antibody, HT1A-1 antibody, OTS8 antibody, PA2.26 antibody, T1A antibody, T1A-2 antibody, OTS-8 antibody, RANDAM-2 antibody, T1-alpha antibody, T1a antibody, T1alpha antibody, E11 antibody, RTI40 antibody, podoplanin antibody, PDPN antibody, Pdpn antibody
- Molecular Weight
- 38-43 kDa
- Gene ID
- 10630, 468675
- UniProt
- Q86YL7
- Pathways
- Dicarboxylic Acid Transport
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