VEGFA antibody
-
- Target See all VEGFA Antibodies
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
-
Reactivity
- Human, Rat, Mouse, Dog, Rabbit
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This VEGFA antibody is un-conjugated
-
Application
- Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-VEGF-A (VEGF/1063), Purified, BSA-free
- Characteristics
- This MAb recognizes proteins of 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Purification
- Purified
- Immunogen
- Human VEGF189 recombinant protein
- Clone
- VEGF-1063
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product VEGFA Primary Antibody
-
-
- Application Notes
-
Immunohistology formalin-fixed 2-4 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
-
Tumor cells in astrocytomas, breast or ovarian carcinomas.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS (no BSA, no azide)
- Preservative
- Azide free
-
- Target
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
- Alternative Name
- VEGF-A (VEGFA Products)
- Synonyms
- MVCD1 antibody, VEGF antibody, VPF antibody, Vegf antibody, Vegf120 antibody, Vegf164 antibody, Vegf188 antibody, Vpf antibody, vegf antibody, vegfa antibody, wu:fj82c06 antibody, VEGF-A antibody, VEGF164 antibody, eVEGF120 antibody, eVEGF164 antibody, vegf-a antibody, vpf antibody, vefg antibody, si:dkey-14d8.3 antibody, wu:fd42e02 antibody, vascular endothelial growth factor A antibody, vascular endothelial growth factor Aa antibody, vascular endothelial growth factor A L homeolog antibody, vascular endothelial growth factor Ab antibody, VEGFA antibody, Vegfa antibody, vegfaa antibody, vegfa.L antibody, vegfa antibody, vegfab antibody
- Background
- MVCD1, Vascular Endothelial Growth Factor A (VEGF-A), Vascular Permeability Factor (VPF), VEGFA
- Molecular Weight
- 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing)
- Gene ID
- 7422, 73793
- UniProt
- P15692
- Pathways
- RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
-