IFNA2 antibody (CF®405S)

Catalog No. ABIN6170879

Product Details anti-IFNA2 Antibody (CF®405S)

Target: IFNA2 (Interferon, alpha 2 (IFNA2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IFNA2 antibody is conjugated to CF®405S

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat), Blocking Peptide (BP), Activity Assay (AcA)

Purpose: Mouse Monoclonal anti-Interferon alpha-2 (N27), CF405S Conjugate

Characteristics: This antibody recognizes a protein of 16-27 kDa, identified as human interferon-i±II (IFN-i±II). Its epitope maps between aa43-53 of the IFN-i±II) (total aa172). This MAb is specific for IFN-i±(II) and does not cross-react with IFN-i±(I). Epitopes of N27 and N39 MAb s are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFN-i±(II) after viral infections. The IFN-i± family consists of 24 or more genes or pseudo-genes. IFN-i±II) is one of the two distinct families (I and II) of human IFN-i±. The i± interferons are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified recombinant human IFN-alpha2

Clone: N27

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry 0.5-1 μg/million cells in 0.1 mL

• Blocking activity order Ab without azide
• Immunofluorescence 0.5-1 μg/mL
• ELISA For coating, order Ab without BSA
• Optimal dilution for a specific application should be determined by user

Comment:

Stimulated human peripheral blood mononuclear cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for IFNA2

Target: IFNA2 (Interferon, alpha 2 (IFNA2))

Alternative Name: Interferon alpha-2 (IFNA2 Products)

Synonyms: IFN-alphaA antibody, IFNA antibody, IFNA2B antibody, INFA2 antibody, Ifa2 antibody, IFNA2 antibody, interferon alpha 2 antibody, interferon, alpha 2 antibody, interferon-alpha-2 antibody, interferon alpha-2 antibody, IFNA2 antibody, Ifna2 antibody, ifna2 antibody, IFN-ALPHA2 antibody, LOC102142148 antibody

Background: Alpha 2a interferon, IFN alpha, IFN-alpha-2, IFNA, IFNA2, IFNA2B, Interferon alpha 2a, Interferon alpha 2b, Interferon alpha-2, Interferon alpha-A, LeIF2, LeIFA

Molecular Weight: 16-27 kDa

Gene ID: 3440

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Hepatitis C