CFTR antibody

Catalog No. ABIN6174638

Product Details anti-CFTR Antibody

Target: CFTR (Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CFTR antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Immunostaining (ISt), Western Blotting (WB)

Purpose: Mouse Monoclonal anti-CFTR CFTR/1342), Purified, with BSA

Characteristics: This antibody recognizes a protein of 165-170 kDa, identified as cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Recombinant human full-length CFTR

Clone: CFTR-1342

Isotype: IgG2a

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

MOLT-4 cells. Pancreas, Kidney or Placenta.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for CFTR

Target: CFTR (Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR))

Alternative Name: CFTR (CFTR Products)

Synonyms: ABC35 antibody, ABCC7 antibody, CF antibody, CFTR/MRP antibody, MRP7 antibody, TNR-CFTR antibody, dJ760C5.1 antibody, CFTR antibody, AW495489 antibody, Abcc7 antibody, RGD1561193 antibody, abc35 antibody, abcc7 antibody, cftr/mrp antibody, mrp7 antibody, tnr-cftr antibody, xcftr antibody, si:dkey-270i2.2 antibody, cystic fibrosis transmembrane conductance regulator antibody, cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) antibody, CFTR antibody, cftr-A antibody, Cftr antibody, cftr antibody

Molecular Weight: 165-170 kDa

Gene ID: 1080