GJB1 antibody (CF®405S)

Catalog No. ABIN6178402

Product Details anti-GJB1 Antibody (CF®405S)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GJB1 antibody is conjugated to CF®405S

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: Mouse Monoclonal anti-Connexin-32 (GJB1/1753), CF405S Conjugate

Characteristics: This Ab recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane ?-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human GJB1 protein

Clone: GJB1-1753

Isotype: IgG

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• Immunofluorescence 1-2 μg/mL Flow cytometry 0.5-1 μg/million cells
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

MCF-7 Cells. Liver, Kidney, Stomach or Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Alternative Name: Connexin-32 (GJB1 Products)

Synonyms: GJIC antibody, cmtx antibody, cmtx1 antibody, connexin-30 antibody, connexin32 antibody, cx30 antibody, cx32 antibody, gja1 antibody, CX32 antibody, GJB1 antibody, AI118175 antibody, Cnx32 antibody, Cx32 antibody, Gjb-1 antibody, CMTX antibody, CMTX1 antibody, gjb1-a antibody, gap junction protein beta 1 antibody, connexin 32 antibody, probable serine/threonine-protein kinase CST antibody, gap junction protein, beta 1 antibody, gap junction protein beta 1 L homeolog antibody, gjb1 antibody, CX32 antibody, LOC9303136 antibody, GJB1 antibody, Gjb1 antibody, gjb1.L antibody

Background: Charcot Marie Tooth neuropathy X linked, CMTX, CMTX1, Connexin-32, Cx32, GAP junction 28  kDa liver protein, Gap junction beta-1 protein, Gap junction protein beta 1 32kD, GJB1

Molecular Weight: 27-32 kDa

Gene ID: 2705, 333303

UniProt: P08034

Pathways: Cell-Cell Junction Organization