SEMA4D/CD100 antibody
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- Target See all SEMA4D/CD100 (SEMA4D) Antibodies
- SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))
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Reactivity
- Human, Baboon
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This SEMA4D/CD100 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF)
- Purpose
- Mouse Monoclonal anti-CD100 (133-1C6), Purified, with BSA
- Characteristics
- This antibody recognizes a homodimeric protein comprised of 50 kDa subunits, identified as CD100 (Workshop VI, Code N-L026). It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Purification
- Purified
- Immunogen
- PHA stimulated human peripheral blood lymphocytes.
- Clone
- 133-1C6
- Isotype
- IgM kappa
- Top Product
- Discover our top product SEMA4D Primary Antibody
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- Application Notes
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Does not react with horse, others not tested
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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Daudi, Raji, HUT-78, Kg1a, U937, and human lymphocytes. Human tonsils and lymph nodes.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 200 μg/mL
- Buffer
- PBS/0.05 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))
- Alternative Name
- CD100 (SEMA4D Products)
- Synonyms
- C9orf164 antibody, CD100 antibody, M-sema-G antibody, SEMAJ antibody, coll-4 antibody, Semacl2 antibody, Semaj antibody, Semcl2 antibody, COLL-4 antibody, semaphorin 4D antibody, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D antibody, Sema4d antibody, SEMA4D antibody
- Background
- COLL4, Collapsin4, GR3, M sema G, MSEMA, SEMA4D, Semacl2, SemaH, SEMAJ, Semaphorin C like 2, Semaphorin H, Semaphorin J, Semaphorin-4D, Semcl2
- Molecular Weight
- 50 kDa (Monomer)
- Gene ID
- 10507, 494406
- UniProt
- Q92854
- Pathways
- Regulation of Cell Size
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