CD11c antibody (AA 637-827) (CF®640R)
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- Target See all CD11c (ITGAX) Antibodies
- CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))
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Binding Specificity
- AA 637-827
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD11c antibody is conjugated to CF®640R
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Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Purpose
- Mouse Monoclonal anti-CD11c (ITGAX/1284), CF640R Conjugate
- Characteristics
- This antibody recognizes a protein of 145 kDa, identified as CD11c. CD11c (ITGAX), a member of the leukointegrin family, shares the same beta subunit with other members of the leukocyte adhesion molecule family, which includes CD11a (LFA-1), CD11b (MAC-1) and CD11d (ITGAD), but has a unique alpha chain. CD11c has been shown to play a role in phagocytosis, cell migration, and cytokine production by monocytes/macrophages as well as induction of T-cell proliferation by Langerhans cells. CD11c is expressed prominently on the plasma membranes of monocytes, tissue macrophages, NK cells, and most dendritic cells (DCs). A lower level of expression is also observed on neutrophils as a result of its high level of expression on most DCs. An antibody to CD11c may aid in identification of lesions with histiocytic origin. It may also been used as a marker for hairy cell leukemia in paraffin-embedded tissues. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human ITGAX protein fragment (aa 637-827) (exact sequence is proprietary)
- Clone
- ITGAX-1284
- Isotype
- IgG2b
- Top Product
- Discover our top product ITGAX Primary Antibody
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- Application Notes
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Immunohistology (formalin) 1-2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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Human dendritic cells. Human lymph nodes and tonsils.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))
- Alternative Name
- CD11c (ITGAX Products)
- Synonyms
- CD11C antibody, SLEB6 antibody, RGD1561123 antibody, AI449405 antibody, Cd11c antibody, Cr4 antibody, N418 antibody, integrin subunit alpha X antibody, integrin alpha X antibody, ITGAX antibody, Itgax antibody
- Background
- CD11 antigen-like family member C|Complement component 3 receptor 4 subunit|Integrin alpha-X|integrin, alpha X (antigen CD11C (p150), alpha polypeptide)|Leu M5 alpha subunit|Leukocyte adhesion glycoprotein p150 95 alpha chain|Myeloid membrane antigen alpha subunit|p150/95
- Molecular Weight
- 145 kDa
- Gene ID
- 3687, 248472
- UniProt
- P20702
- Pathways
- Complement System, Activated T Cell Proliferation, Integrin Complex
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