CDX2 antibody (AA 150-249) (CF®488A)
-
- Target See all CDX2 Antibodies
- CDX2 (Caudal Type Homeobox 2 (CDX2))
-
Binding Specificity
- AA 150-249
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This CDX2 antibody is conjugated to CF®488A
-
Application
- ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)
- Purpose
- Mouse Monoclonal anti-CDX2 (CDX2/1690), CF488A Conjugate
- Characteristics
- The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoids and is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma. However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human CDX2 protein fragment (aa150-249) (exact sequence is proprietary)
- Clone
- CDX2-1690
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product CDX2 Primary Antibody
-
-
- Application Notes
-
ELISA 2-4 μg/mL for coating (order Ab without BSA)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
-
HT29 cells or Colon Carcinoma
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
-
- Target
- CDX2 (Caudal Type Homeobox 2 (CDX2))
- Alternative Name
- CDX2 (CDX2 Products)
- Synonyms
- CDX2 antibody, MGC130987 antibody, cdx3-like antibody, CDX-3 antibody, CDX3 antibody, CDX-2 antibody, Cdx-2 antibody, caudal type homeobox 2 antibody, caudal type homeobox 2 L homeolog antibody, caudal type homeo box 2 antibody, CDX2 antibody, cdx2.L antibody, Cdx2 antibody
- Background
- Caudal type homeobox 2, Caudal type homeobox transcription factor 2, Caudal-type homeobox protein 2, CDX2
- Molecular Weight
- 40 kDa
- Gene ID
- 1045, 174249
- UniProt
- Q99626
- Pathways
- Peptide Hormone Metabolism, Stem Cell Maintenance
-