Transferrin Receptor antibody (AA 94-212) (CF®405S)
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- Target See all Transferrin Receptor (TFRC) Antibodies
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
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Binding Specificity
- AA 94-212
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Transferrin Receptor antibody is conjugated to CF®405S
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Coating (Coat)
- Purpose
- Mouse Monoclonal anti-CD71 / Transferrin Receptor (TFRC/1818), CF405S Conjugate
- Characteristics
- This antibody recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human TFRC protein fragment (aa94-212) (exact sequence is proprietary)
- Clone
- TFRC-1818
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product TFRC Primary Antibody
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- Application Notes
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Flow cytometry 0.5-1 μg/million cells in 0.1 mL
- Immunohistology (formalin) 1-2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- ELISA 2-4 μg/mL for coating (order Ab without BSA)
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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PC3 cells. Human placenta or breast carcinoma.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
- Alternative Name
- CD71 / Transferrin Receptor (TFRC Products)
- Synonyms
- Trfr antibody, LOC100219280 antibody, tfr antibody, cd71 antibody, tfr1 antibody, trfr antibody, CD71 antibody, T9 antibody, TFR antibody, TFR1 antibody, TR antibody, TRFR antibody, p90 antibody, 2610028K12Rik antibody, AI195355 antibody, AI426448 antibody, AU015758 antibody, E430033M20Rik antibody, Mtvr-1 antibody, Mtvr1 antibody, transferrin receptor antibody, TFRC antibody, Tfrc antibody, tfrc antibody
- Background
- Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR
- Molecular Weight
- 85-95 kDa (monomer), 190 kDa (dimer)
- Gene ID
- 7037, 529618
- UniProt
- P02786
- Pathways
- Transition Metal Ion Homeostasis
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