Nestin antibody (C-Term)

Catalog No. ABIN6242479

Product Details anti-Nestin Antibody

Target: Nestin (NES)

Binding Specificity: AA 1560-1590, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Nestin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1560~1590 amino acids from the C-terminal region of human nestin.

Clone: RB2857

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-Nestin antibody (ABIN6242479)

Chen, Ou, Song, Xian, Ouyang, Xie, Xue, Sun: "Generation of integration-free induced pluripotent stem cells (GZHMUi001-A) by reprogramming peripheral blood mononuclear cells from a 47, XXX syndrome patient." in: Stem cell research, Vol. 23, pp. 57-60, (2018) (PubMed).

May, Sureban, Lightfoot, Hoskins, Brackett, Postier, Ramanujam, Rao, Wyche, Anant, Houchen: "Identification of a novel putative pancreatic stem/progenitor cell marker DCAMKL-1 in normal mouse pancreas." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 2, pp. G303-10, (2010) (PubMed).

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin (NES Products)

Synonyms: paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody

Background: Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.

Molecular Weight: 177439

NCBI Accession: NP_006608

UniProt: P48681