Thrombomodulin antibody (AA 69-194)

Catalog No. ABIN6251861

Product Details anti-Thrombomodulin Antibody

Target: Thrombomodulin (THBD)

Binding Specificity: AA 69-194

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thrombomodulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: Human recombinant partial protein corresponding to amino acids 69-194 was used as the immunogen for this Thrombomodulin antibody.

Clone: THBD-1782

Isotype: IgG2a kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/10^6 cells in 0.1ml

IF: 0.5-1 μg/mL

IHC (FFPE): 1-2 μg/mL for 30 min at RT

ELISA: 2-4 μg/mL, order the BSA-free formulation for coating

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thrombomodulin antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Thrombomodulin

Target: Thrombomodulin (THBD)

Alternative Name: Thrombomodulin (THBD Products)

Synonyms: AHUS6 antibody, BDCA3 antibody, CD141 antibody, THPH12 antibody, THRM antibody, TM antibody, AI385582 antibody, THBD antibody, HAST antibody, HAST3 antibody, M-PST antibody, ST1A3/ST1A4 antibody, ST1A5 antibody, STM antibody, TL-PST antibody, thrombomodulin antibody, sulfotransferase family 1A member 3 antibody, THBD antibody, Thbd antibody, SULT1A3 antibody

Background: It recognizes a protein of 75  kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.

Gene ID: 7056

UniProt: P07204