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EpCAM antibody (Extracellular Domain)
EPCAM
Reactivity: Human
WB, FACS, IF, ELISA, IHC (p)
Host: Mouse
Monoclonal
EGP40-1798
unconjugated
Product Details anti-EpCAM Antibody
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Target
See all EpCAM (EPCAM) Antibodies
EpCAM (EPCAM)
(Epithelial Cell Adhesion Molecule (EPCAM))
Binding Specificity
All epitopes for EpCAM antibodies
AA 77-202, Extracellular Domain
Reactivity
All reactivities for EpCAM antibodies
Human
Host
All hosts for EpCAM antibodies
Mouse
Clonality
All clonalities for EpCAM antibodies
Monoclonal
Conjugate
All conjugates for EpCAM antibodies
This EpCAM antibody is un-conjugated
Application
All applications for EpCAM antibodies
Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
Protein G affinity chromatography
Immunogen
A human recombinant partial protein corresponding to amino acids 77-202 (extracellular domain) was used as the immunogen for the Ep-CAM antibody.
Clone
EGP40-1798
Isotype
IgG2b kappa
Top Product
Discover our top product EPCAM Primary Antibody
Alternatives
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Application Details
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Application Notes
ELISA: 2-4 μg/mL, order the BSA free formulation for coating WB: 0.5-1 μg/mL IF: 1-2 μg/mL Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT Flow Cytometry: 0.5-1 μg/million cells in 0.1ml Titering of the Ep-CAM antibody may be required for optimal performance.
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Target Details for EpCAM
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Target
EpCAM (EPCAM)
(Epithelial Cell Adhesion Molecule (EPCAM))
Alternative Name
EpCAM (EPCAM Products )
Synonyms
DIAR5 antibody, EGP-2 antibody, EGP314 antibody, EGP40 antibody, ESA antibody, HNPCC8 antibody, KS1/4 antibody, KSA antibody, M4S1 antibody, MIC18 antibody, MK-1 antibody, TACSTD1 antibody, TROP1 antibody, ECS-1 antibody, ECS1 antibody, ESA1 antibody, M17S1 antibody, EGP antibody, Ep-CAM antibody, sb:cb6 antibody, tacstd antibody, wu:fj17g02 antibody, zgc:110304 antibody, zgc:77119 antibody, tacstd1 antibody, MGC80540 antibody, EPCAM antibody, egp antibody, ksa antibody, m4s1 antibody, mk-1 antibody, cd326 antibody, egp40 antibody, mic18 antibody, trop1 antibody, ep-cam antibody, hegp-2 antibody, co17-1a antibody, ga733-2 antibody, CD326 antibody, Egp314 antibody, EpCAM1 antibody, GA733-2 antibody, Ly74 antibody, Tacsd1 antibody, Tacstd1 antibody, gp40 antibody, epithelial cell adhesion molecule antibody, flotillin 2 antibody, epithelial cell adhesion molecule S homeolog antibody, EPCAM antibody, FLOT2 antibody, epcam antibody, epcam.S antibody, Epcam antibody
Background
EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Gene ID
4072
UniProt
P16422
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