KRT1 antibody
-
- Target See all KRT1 Antibodies
- KRT1 (Cytokeratin HMW (KRT1))
-
Reactivity
- Human, Rat, Mouse
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This KRT1 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)
- Purification
- Protein G affinity chromatography
- Immunogen
- Solubilized keratin extract from human stratum corneum was used as the immunogen for this HMW Cytokeratin antibody.
- Clone
- 34BE12
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product KRT1 Primary Antibody
-
-
- Application Notes
-
Flow Cytometry: 0.5-1 μg/10e6 cells
IF: 0.5-1.0 μg/mL
WB: 0.5-1.0 μg/mL
IHC (FFPE): 0.5-1.0 μg/mL for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HMW Cytokeratin antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
-
- Target
- KRT1 (Cytokeratin HMW (KRT1))
- Alternative Name
- HMW Cytokeratin (KRT1 Products)
- Synonyms
- CK1 antibody, EHK antibody, EHK1 antibody, EPPK antibody, K1 antibody, KRT1A antibody, NEPPK antibody, keratin 1 antibody, KRT1 antibody
- Background
- This antibody recognizes HMW Keratin 1, 5, 10 and 14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. The HMW Cytokeratin antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this mAb. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. It has also been useful in separating benign from malignant intraductal breast proliferations.
- Gene ID
- 3848
- UniProt
- P04264
-