Thyroglobulin antibody
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- Target See all Thyroglobulin (TG) Antibodies
- Thyroglobulin (TG)
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Reactivity
- Human, Mouse, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Thyroglobulin antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Flow Cytometry (FACS)
- Purification
- Protein G affinity chromatography
- Immunogen
- Human thyroid follicular cells were used as the immunogen for this Thyroglobulin antibody.
- Clone
- 2H11 or TGB04
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product TG Primary Antibody
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- Application Notes
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Flow Cytometry: 0.5-1 μg/million cells
IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Target
- Thyroglobulin (TG)
- Alternative Name
- Thyroglobulin (TG Products)
- Synonyms
- TG antibody, cb717 antibody, AITD3 antibody, TGN antibody, Tgn antibody, cog antibody, thyroglobulin antibody, TG antibody, tg antibody, LOC701526 antibody, LOC701756 antibody, LOC100434297 antibody, Tg antibody
- Background
- Thyroglobulin is a 660 kDa dimeric pre-protein with mutiple glycosylation sites, detected at ~300 kDa in western blot. It is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimers, thyroglobulin monomers undergo conformational maturation in the endoplasmic reticulation. The vast majority of follicular carcinomas of the thyroid will give positive immunoreactivity for thyroglobulin antibody even though sometimes only focally. Poorly differentiated carcinomas of the thyroid are frequently thyroglobulin antibody negative. Adenocarcinomas of other-than-thyroid origin do not react with this antibody. This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of thyroglobulin in metastatic lesions establishes the thyroid origin of tumor. Thyroglobulin antibody, combined with calcitonin antibody, can identify medullary carcinomas of the thyroid. Furthermore, thyroglobulin antibody, combined with TTF1 antibody, can be a reliable marker to differentiate between primary thyroid and lung neoplasms.
- Gene ID
- 7038
- UniProt
- P01266
- Pathways
- Thyroid Hormone Synthesis
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