KRT14 antibody
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- Target See all KRT14 Antibodies
- KRT14 (Keratin 14 (KRT14))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This KRT14 antibody is un-conjugated
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Application
- Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogen
- Recombinant full-length human KRT14 protein was used as the immunogen for the Keratin 14 antibody.
- Clone
- CTKN14-1
- Isotype
- IgG3
- Top Product
- Discover our top product KRT14 Primary Antibody
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- Application Notes
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Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Optimal dilution of the Keratin 14 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Target
- KRT14 (Keratin 14 (KRT14))
- Alternative Name
- Keratin 14 (KRT14 Products)
- Synonyms
- CK14 antibody, EBS3 antibody, EBS4 antibody, K14 antibody, NFJ antibody, AI626930 antibody, Krt-1.14 antibody, Krt1-14 antibody, Ka14 antibody, ouro2 antibody, keratin 14 antibody, keratin 14, type I L homeolog antibody, KRT14 antibody, Krt14 antibody, krt14.L antibody
- Background
- Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.
- Gene ID
- 3861
- UniProt
- P02533
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