Calponin antibody

Catalog No. ABIN6252459

Product Details anti-Calponin Antibody

Target: Calponin

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calponin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS)

Purification: Protein G affinity chromatography

Immunogen: Crude human uterus extract was used as the immunogen for the Calponin antibody.

Clone: CALP

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Optimal dilution of the Calponin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Calponin

Target: Calponin

Abstract: Calponin Products

Synonyms: CalPoNin antibody, cpn-1 antibody, cpn-2 antibody

Background: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34  kDa form and a 29  kDa form. Expression of the 29  kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34  kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Gene ID: 1264

UniProt: P51911