Filaggrin antibody (AA 998-1104)

Catalog No. ABIN6252502

Product Details anti-Filaggrin Antibody

Target: Filaggrin (FLG)

Binding Specificity: AA 998-1104

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Filaggrin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Purification: Protein G affinity chromatography

Immunogen: Amino acids 998-1104 of human FLG were used as the immunogen for this Filaggrin antibody.

Clone: FLG-1561

Isotype: IgG

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/10^6 cells in 0.1ml

IF: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 min at RT

Optimal dilution of the Filaggrin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Filaggrin

Target: Filaggrin (FLG)

Alternative Name: Filaggrin (FLG Products)

Synonyms: AW107830 antibody, ft antibody, FLG antibody, ATOD2 antibody, Pflg antibody, Flg antibody, filaggrin antibody, Flg antibody, FLG antibody, LOC100351762 antibody, LOC100728006 antibody

Background: Filaggrin (FLG) is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple Filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional Filaggrin molecules. Active Filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Gene ID: 2312

UniProt: P20930

Pathways: Sensory Perception of Sound, Stem Cell Maintenance