CA9 antibody

Catalog No. ABIN6252593

Product Details anti-CA9 Antibody

Target: CA9 (Carbonic Anhydrase IX (CA9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CA9 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the CAIX antibody.

Clone: CBAD9-1

Isotype: IgG2b kappa

Application Details

Application Notes: Western blot: 1-2 μg/mL



Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Titering of the CAIX antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for CA9

Target: CA9 (Carbonic Anhydrase IX (CA9))

Alternative Name: CAIX (CA9 Products)

Synonyms: CA9 antibody, CAIX antibody, Ca9 antibody, MN/CA9 antibody, MN antibody, carbonic anhydrase 9 antibody, CA9 antibody, CAH9 antibody, Car9 antibody

Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. Carbonic anhydrase IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. [RefSeq]

Gene ID: 768

UniProt: Q16790