Topoisomerase II alpha antibody (AA 1352-1493)

Catalog No. ABIN6252772

Product Details anti-Topoisomerase II alpha Antibody

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Binding Specificity: AA 1352-1493

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purification: Protein G affinity chromatography

Immunogen: Amino acids 1352-1493 from the human protein were used as the immunogen for this Topoisomerase II alpha antibody.

Clone: TOP2A-1361

Isotype: IgG2b kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/10^6 cells in 0.1ml

IF: 1-2 μg/mL

Western blot: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A (TOP2A Products)

Synonyms: TOP2 antibody, TP2A antibody, TOP2A antibody, Top-2 antibody, TOP-2 antibody, F12B17.110 antibody, F12B17_110 antibody, thimet metalloendopeptidase 2 antibody, fc08c07 antibody, wu:fc08c07 antibody, DNA topoisomerase II alpha antibody, DNA topoisomerase II, alpha isozyme antibody, topoisomerase (DNA) II alpha antibody, Zincin-like metalloproteases family protein antibody, TOP2A antibody, Bm1_08705 antibody, Top2a antibody, AT5G10540 antibody, top2a antibody

Background: This mAb recognizes an ~170  kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Gene ID: 7153

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases