ATF6 antibody (Internal Region)

Catalog No. ABIN6254886

Product Details anti-ATF6 Antibody

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: ATF6 Antibody detects endogenous levels of total ATF6.

Predicted Reactivity: Pig,Zebrafish,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ATF6, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-ATF6 antibody (ABIN6254886)

Lu, Chen, Tang, Wang, Zhang: "GRP78 silencing enhances hyperoxia-induced alveolar epithelial cell apoptosis via CHOP pathway." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 1493-1501, (2018) (PubMed).

Chen, Li, Liu, Luo, Liao, Li, Liu, Cheng, Lu, Chen: "Oleic acid protects saturated fatty acid mediated lipotoxicity in hepatocytes and rat of non-alcoholic steatohepatitis." in: Life sciences, Vol. 203, pp. 291-304, (2018) (PubMed).

Yang, He, Li, Zhang, He, Duan, Huang, Xiang: "SWNHs (Single-Wall Carbon Nanohorns) Supervises Endoplasmic Reticulum (ER) Stress in Hepatocellular Carcinoma." in: Journal of nanoscience and nanotechnology, Vol. 18, Issue 10, pp. 6740-6745, (2018) (PubMed).

Target Details for ATF6

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Alternative Name: ATF6 (ATF6 Products)

Synonyms: ATF-6-like antibody, GB16435 antibody, ATF6 antibody, Atf6 antibody, ATF6A antibody, 9130025P16Rik antibody, 9630036G24 antibody, AA789574 antibody, Atf6alpha antibody, ESTM49 antibody, activating transcription factor 6 antibody, uncharacterized LOC412432 antibody, activating transcription factor 6 S homeolog antibody, cyclic AMP-dependent transcription factor ATF-6 alpha antibody, ATF6 antibody, LOC412432 antibody, atf6.S antibody, LOC100122162 antibody, Atf6 antibody

Background:

Description: Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N9-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina.

Gene: ATF6

Molecular Weight: 75kDa

Gene ID: 22926

UniProt: P18850

Pathways: ER-Nucleus Signaling, Unfolded Protein Response