JNK antibody (pThr183, pTyr185)

Catalog No. ABIN6255620

Product Details anti-JNK Antibody

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Binding Specificity: pThr183, pTyr185

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JNK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Phospho-JNK1/2/3 (Thr183+Tyr185) Antibody detects endogenous levels of JNK1/2/3 only when phosphorylated at Threonine 183+Tyrosine 185

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human JNK1/2/3 around the phosphorylation site of Threonine 183+Tyrosine 185

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:100-1:500 IF 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

References for anti-JNK antibody (ABIN6255620)

Peng, Wu, Deng, Zhou, Song, Yang, Zhang, Xu, Xia, Cai, Liu, Peng: "MiR-377 promotes white adipose tissue inflammation and decreases insulin sensitivity in obesity via suppression of sirtuin-1 (SIRT1)." in: Oncotarget, Vol. 8, Issue 41, pp. 70550-70563, (2018) (PubMed).

Li, Zhang, Jin, Zou, Wang, Hao, Fu, Jiao, Zhang, Lin, Matsuzaki, Zhao: "Dysifragilone A inhibits LPS‑induced RAW264.7 macrophage activation by blocking the p38 MAPK signaling pathway." in: Molecular medicine reports, Vol. 17, Issue 1, pp. 674-682, (2018) (PubMed).

Geng, Yang, Wang, Zhang, Hu, Gu, Li: "Trimethylamine N-oxide promotes atherosclerosis via CD36-dependent MAPK/JNK pathway." in: Biomedicine & pharmacotherapy, Vol. 97, pp. 941-947, (2018) (PubMed).

Zhang, Fei, Tao, Li, Shen, Wang, Liu, Xu: "Neuroprotective Effect of Modified Xijiao Dihuang Decoction against Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury in PC12 Cells: Involvement of TLR4-MyD88/NF-κB Signaling Pathway." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 3848595, (2018) (PubMed).

Ji, Yuan, Yuan, Xia, Yang: "The p75 neurotrophin receptor might mediate sepsis-induced synaptic and cognitive impairments." in: Behavioural brain research, Vol. 347, pp. 339-349, (2018) (PubMed).

Tang, Bai, Zou, Meng, Xia, Cheng, Mu, Zhou, Wang, Qin, Cao, Jiang, Chen: "Ferroptosis is newly characterized form of neuronal cell death in response to arsenite exposure." in: Neurotoxicology, Vol. 67, pp. 27-36, (2018) (PubMed).

Zhang, Luan, Li, Liu, Liu, Wang, Li, Wang, Zou, Liu, Matsuzaki, Zhao: "Anti-inflammatory action of ambuic acid, a natural product isolated from the solid culture of Pestalotiopsis neglecta, through blocking ERK/JNK mitogen-activated protein kinase signaling pathway." in: Experimental and therapeutic medicine, Vol. 16, Issue 2, pp. 1538-1546, (2018) (PubMed).

Jin, Han, Yang, Hu, Liu, Zhao: "11-O-acetylcyathatriol inhibits MAPK/p38-mediated inflammation in LPS-activated RAW 264.7 macrophages and has a protective effect on ethanol-induced gastric injury." in: Molecular medicine reports, Vol. 14, Issue 1, pp. 874-80, (2017) (PubMed).

Jin, Song, Wang, Zhang, Qiu, Zhao: "Tiliroside, the major component of Agrimonia pilosa Ledeb ethanol extract, inhibits MAPK/JNK/p38-mediated inflammation in lipopolysaccharide-activated RAW 264.7 macrophages." in: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 499-505, (2017) (PubMed).

Geng, Zhao, Liang, Zhang, Xie, Bi, Wang, Zhang, Cheng, Yu, Zhong: "Cigarette smoke extract-induced proliferation of normal human urothelial cells via the MAPK/AP-1 pathway." in: Oncology letters, Vol. 13, Issue 1, pp. 469-475, (2017) (PubMed).

Target Details for JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Alternative Name: JNK1/2/3 (MAPK8 Products)

Synonyms: JNK antibody, JNK-46 antibody, JNK1 antibody, JNK1A2 antibody, JNK21B1/2 antibody, PRKM8 antibody, SAPK1 antibody, SAPK1c antibody, AI849689 antibody, Prkm8 antibody, BSK antibody, BSK/DJNK antibody, Bsk antibody, CG5680 antibody, D-JNK antibody, D-junk antibody, DBSK/JNK antibody, DJNK antibody, DJNK/bsk antibody, Dmel\\CG5680 antibody, JNK/SAPK antibody, Jnk antibody, Junk antibody, SAPKa antibody, c-Jun antibody, dJNK antibody, jnk antibody, T10F20.15 antibody, jnk1 antibody, sapk1 antibody, mapk8 antibody, zgc:112379 antibody, JNKb antibody, mapk8b antibody, mitogen-activated protein kinase 8 antibody, Stress-activated protein kinase jnk-1 antibody, basket antibody, Protein kinase superfamily protein antibody, mitogen-activated protein kinase 8 L homeolog antibody, mitogen-activated protein kinase 8b antibody, mitogen-activated protein kinase 8B-like antibody, MAPK8 antibody, jnk-1 antibody, Mapk8 antibody, bsk antibody, ATMPK8 antibody, mapk8.L antibody, mapk8b antibody, LOC109094263 antibody

Background:

Description: Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed:10747973).

Gene: MAPK8

Molecular Weight: 46,54kDa

Gene ID: 5599

UniProt: P45983, P45984, P53779

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins