ATG4A antibody (N-Term)

Catalog No. ABIN6278139

Product Details anti-ATG4A Antibody

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: ATG4A Antibody detects endogenous levels of total ATG4A.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ATG4A, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ATG4A

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Alternative Name: ATG4A (ATG4A Products)

Synonyms: apg4a antibody, autl2 antibody, F6E13.27 antibody, APG4A antibody, zgc:111958 antibody, Aut2a antibody, AUTL2 antibody, AI627006 antibody, AV169859 antibody, Apg4a antibody, Atg4al antibody, Autl2 antibody, Atg4a antibody, autophagy related 4A cysteine peptidase L homeolog antibody, autophagy related 4A cysteine peptidase antibody, Peptidase family C54 protein antibody, ATG4 autophagy related 4 homolog A (S. cerevisiae) antibody, autophagy related 4A, cysteine peptidase antibody, atg4a.L antibody, ATG4A antibody, AT2G44140 antibody, atg4a antibody, Atg4a antibody

Background:

Description: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Gene: ATG4A

Molecular Weight: 45 kDa

Gene ID: 115201

UniProt: Q8WYN0

Pathways: Autophagy