BNIP3 antibody (C-Term)

Catalog No. ABIN6260296

Product Details anti-BNIP3 Antibody

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BNIP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: BNIP3 Antibody detects endogenous levels of total BNIP3.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human BNIP3, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for BNIP3

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Alternative Name: BNIP3 (BNIP3 Products)

Synonyms: NIP3 antibody, Nip3 antibody, BCL2 interacting protein 3 antibody, BCL2/adenovirus E1B interacting protein 3 antibody, BNIP3 antibody, Bnip3 antibody

Background:

Description: Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.

Gene: BNIP3

Molecular Weight: 30 kDa

Gene ID: 664

UniProt: Q12983

Pathways: Autophagy, Brown Fat Cell Differentiation