GRLF1 antibody (Internal Region)
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- Target See all GRLF1 Antibodies
- GRLF1 (Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GRLF1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificity
- GRF-1 Antibody detects endogenous levels of total GRF-1.
- Predicted Reactivity
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human GRF-1, corresponding to a region within the internal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product GRLF1 Primary Antibody
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- Application Notes
- WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- GRLF1 (Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1))
- Alternative Name
- ARHGAP35 (GRLF1 Products)
- Synonyms
- GRF-1 antibody, GRLF1 antibody, P190-A antibody, P190A antibody, p190ARhoGAP antibody, p190RhoGAP antibody, 6430596G11Rik antibody, AI841135 antibody, Arhgap35 antibody, mKIAA1722 antibody, p190A antibody, Grlf1 antibody, p190-A antibody, arhgap35 antibody, grf-1 antibody, grlf1 antibody, p190-a antibody, p190a antibody, p190arhogap antibody, p190rhogap antibody, Rho GTPase activating protein 35 antibody, Rho GTPase activating protein 35 L homeolog antibody, ARHGAP35 antibody, Arhgap35 antibody, arhgap35.L antibody
- Background
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Description: Rho GTPase-activating protein (GAP) (PubMed:19673492, PubMed:28894085). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed:19673492). This binding is inhibited by phosphorylation by PRKCA (PubMed:19673492). Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (By similarity). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (By similarity). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (By similarity). Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (By similarity). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (By similarity). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3', this function is however unclear and would need additional experimental evidences (PubMed:1894621).
Gene: ARHGAP35
- Molecular Weight
- 155kDa
- Gene ID
- 2909
- UniProt
- Q9NRY4
- Pathways
- Tube Formation
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