ROBO1 antibody (Internal Region)

Catalog No. ABIN6279788

Product Details anti-ROBO1 Antibody

Target: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROBO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: ROBO1 Antibody detects endogenous levels of total ROBO1.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ROBO1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ROBO1

Target: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Alternative Name: ROBO1 (ROBO1 Products)

Synonyms: DUTT1 antibody, SAX3 antibody, AW494633 antibody, AW742721 antibody, Gm310 antibody, ROBO1 antibody, dutt1 antibody, roundabout antibody, sax3 antibody, MGC122264 antibody, roundabout-1 antibody, roundabout guidance receptor 1 antibody, roundabout, axon guidance receptor, homolog 1 (Drosophila) antibody, roundabout guidance receptor 1 L homeolog antibody, ROBO1 antibody, Robo1 antibody, robo1 antibody, robo1.L antibody

Background:

Description: Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed:10102268, PubMed:24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B, inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed:26529257). May be required for lung development (By similarity).

Gene: ROBO1

Molecular Weight: 181 kDa

Gene ID: 6091

UniProt: Q9Y6N7

Pathways: Positive Regulation of Endopeptidase Activity