RPL5 antibody (Internal Region)
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- Target See all RPL5 Antibodies
- RPL5 (Ribosomal Protein L5 (RPL5))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RPL5 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- RPL5 Antibody detects endogenous levels of total RPL5.
- Predicted Reactivity
- Bovine,Horse,Dog,Chicken,Xenopus
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human RPL5, corresponding to a region within the internal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product RPL5 Primary Antibody
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- Application Notes
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- RPL5 (Ribosomal Protein L5 (RPL5))
- Alternative Name
- RPL5 (RPL5 Products)
- Synonyms
- DBA6 antibody, L5 antibody, U21RNA antibody, CG17489 antibody, Dmel\\CG17489 antibody, E-2d antibody, M(2)40B antibody, Rp L5 antibody, Rpl5 antibody, dRPL5 antibody, yip6 antibody, rpl5 antibody, wu:fj02g05 antibody, zgc:86854 antibody, mg:cb01f08 antibody, rpl5l antibody, zgc:56511 antibody, zgc:77176 antibody, ribosomal protein L5 antibody, 60S ribosomal protein L5 antibody, Ribosomal protein L5 antibody, ribosomal protein L5 L homeolog antibody, 50S ribosomal protein L5 antibody, ribosomal protein L5 S homeolog antibody, ribosomal protein L5b antibody, rpl5 antibody, ribosomal protein L5a antibody, Rpl5 antibody, RPL5 antibody, rpl-5 antibody, RpL5 antibody, rpl5.L antibody, rpl5 antibody, rpl5.S antibody, rpl5b antibody, rpl5a antibody
- Background
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Description: Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs (PubMed:12962325, PubMed:19061985, PubMed:24120868, PubMed:23636399). It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53 (PubMed:24120868).
Gene: RPL5
- Molecular Weight
- 34kDa
- Gene ID
- 6125
- UniProt
- P46777
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