ZIC1/2/3 antibody

Catalog No. ABIN6266105

Product Details anti-ZIC1/2/3 Antibody

Target: ZIC1/2/3 (Zic1/2/3/4/5) (Zic Family Member 1/2/3/4/5 (Zic1/2/3/4/5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZIC1/2/3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: ZIC1/2/3 antibody detects endogenous levels of total ZIC1/2/3

Cross-Reactivity: Human, Mouse (Murine)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ZIC1/2/3

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:3000 IHC: 1:50~1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for ZIC1/2/3

Target: ZIC1/2/3 (Zic1/2/3/4/5) (Zic Family Member 1/2/3/4/5 (Zic1/2/3/4/5))

Alternative Name: ZIC1/2/3 (Zic1/2/3/4/5 Products)

Background:

Description: Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally-located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3' (By similarity).

Gene: ZIC1

Molecular Weight: 51kDa

Gene ID: 7545

UniProt: Q15915, O95409, O60481