E2F4 antibody

Catalog No. ABIN6266531

Product Details anti-E2F4 Antibody

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: E2F4 antibody detects endogenous levels of total E2F4

Cross-Reactivity: Human, Monkey, Mouse (Murine), Rat (Rattus)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human E2F4

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:3000 IHC: 1:50~1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for E2F4

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Alternative Name: E2F4 (E2F4 Products)

Synonyms: E2F-4 antibody, 2010111M04Rik antibody, AI427446 antibody, E2F4 antibody, e2f-4 antibody, fb72f07 antibody, wu:fb72f07 antibody, wu:fe05f06 antibody, zgc:63815 antibody, E2F transcription factor 4 antibody, E2F transcription factor 4 S homeolog antibody, E2F4 antibody, E2f4 antibody, e2f4.S antibody, e2f4 antibody

Background:

Description: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.

Gene: E2F4

Molecular Weight: 44kDa

Gene ID: 1874

UniProt: Q16254, Q15329

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size