FAS antibody (AA 230-310)

Catalog No. ABIN6282381

Product Details anti-FAS Antibody

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Binding Specificity: AA 230-310

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: FAS is a protein encoded by the FAS gene which is approximately 37,7 kDa. FAS isoform 1 is localised to the cell membrane with other isoforms being secreted. It is involved in dimerization of procaspase-8, allograft rejection, apoptosis modulation and signalling, PEDF induced signalling and the TNFR1 pathway. This protein falls under the TNF-receptor superfamily. This receptor contains a death domain and plays a central role in the physiological regulation of programmed cell death, it has also been implicated in the pathogenesis of various malignancies and diseases of the immune system. FAS isoform 1 and 6 is expressed in resting peripheral blood mononuclear cells. Mutations in the FAS gene may result in autoimmune lymphoproliferative syndrome. STJ93041 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of FAS protein.

Specificity: FAS Polyclonal Antibody detects endogenous levels of FAS protein.

Characteristics: Rabbit polyclonal to FAS.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human FAS around the non-phosphorylation site of Y291.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000

Comment:

Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: FAS (FAS Products)

Synonyms: ALPS1A antibody, APO-1 antibody, APT1 antibody, CD95 antibody, FAS1 antibody, FASTM antibody, TNFRSF6 antibody, AI196731 antibody, APO1 antibody, TNFR6 antibody, Tnfrsf6 antibody, lpr antibody, Fas cell surface death receptor antibody, Fas (TNF receptor superfamily member 6) antibody, FAS antibody, Fas antibody, fas antibody

Molecular Weight: 42 kDa

Gene ID: 355

UniProt: P25445

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity