CaMKIIbeta/gamma/delta antibody (pThr287)

Catalog No. ABIN6282451

Product Details anti-CaMKIIbeta/gamma/delta Antibody

Target: CaMKIIbeta/gamma/delta

Binding Specificity: AA 230-310, pThr287

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CaMKIIbeta/gamma/delta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: CaMKIIβ/γ/δ is a protein encoded by the CAMK2B gene which is approximately 72,6 kDa. CaMKIIβ/γ/δ is localised to the cytoplasm. It is involved in RET signalling, post NMDA receptor activation events and cardiac conduction. It is a calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation. It plays a role in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. CaMKIIβ/γ/δ is widely expressed in human tissue including the adult and foetal brain. The CAMK2B gene is associated with Phencyclidine abuse. STJ90941 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous CaMKIIβ/γ/δ protein which only binds about T287 when T287 is phosphorylated.

Specificity: Phospho-CaMKIIβ/γ/δ (T287) Polyclonal Antibody detects endogenous levels of CaMKIIβ/γ/δ protein only when phosphorylated at T287.

Characteristics: Rabbit polyclonal to Phospho-CaMKIIβ/γ/δ (T287).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human CaMKIIbeta/gamma/delta around the phosphorylation site of T287.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000

Comment:

Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for CaMKIIbeta/gamma/delta

Target: CaMKIIbeta/gamma/delta

Abstract: CaMKIIbeta/gamma/delta Products

Molecular Weight: 50/65 kDa

Gene ID: 816

UniProt: Q13554