Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purpose
VCP is a protein encoded by the VCP gene which is approximately 89,3 kDa. VCP is localised to the cytoplasm, endoplasmic reticulum and nucleus. It is involved in CDK-mediated phosphorylation and removal of Cdc6, the innate immune system, the cellular response to heat stress and the HIV life cycle. It is a structural protein that associates with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. VCP is expressed in the nervous system, lung, liver, blood and skin. Mutations in the VCP gene result in inclusion body myopathy with early-onset Paget and Charcot-Marie-tooth disease type 2. STJ96231 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope specific immunogen. This polyclonal antibody detects endogenous levels of VCP protein.
Specificity
VCP Polyclonal Antibody detects endogenous levels of VCP protein.
Characteristics
Rabbit polyclonal to VCP.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
Synthesized peptide derived from human VCP around the non-phosphorylation site of S352.